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Selective isolation of vancomycin-resistant enterococci

K G van Horn1, C A Gedris, K M Rodney

  • 1Department of Clinical Pathology, Westchester County Medical Center, Valhalla, New York 10595, USA.

Journal of Clinical Microbiology
|April 1, 1996
PubMed
Summary
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This study found that Enterococcel broth supplemented with vancomycin effectively isolates vancomycin-resistant enterococci (VRE) from patient samples. This method provides rapid and selective detection of VRE, crucial for infection control.

Area of Science:

  • Clinical Microbiology
  • Infectious Diseases
  • Antimicrobial Resistance

Background:

  • Vancomycin-resistant enterococci (VRE) pose a significant threat in healthcare settings.
  • Rapid and accurate detection of VRE is essential for effective infection control and patient management.

Purpose of the Study:

  • To evaluate the efficacy of two modified broth formulations, Enterococcel and M-Enterococcus, supplemented with vancomycin for the selective isolation of VRE.
  • To compare the performance of vancomycin-supplemented Enterococcel and M-Enterococcus broths in detecting VRE from clinical surveillance specimens.

Main Methods:

  • Two selective broth media, Enterococcel and M-Enterococcus, were supplemented with 6 µg/mL vancomycin.
  • Broths were tested against various bacterial species including Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, and different strains of enterococci (vancomycin-susceptible and resistant).

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  • The broths were also evaluated using 193 perianal specimens from patients at risk for VRE colonization.

    • Main Results:

    • Both vancomycin-supplemented broths successfully detected as few as 1-9 CFU of VRE while inhibiting the growth of other tested organisms.
    • Enterococcel-vancomycin broth identified VRE in 66 perianal swab cultures, while M-Enterococcus-vancomycin broth identified VRE in 62 cultures.
    • Enterococcel-vancomycin broth demonstrated earlier detection of VRE, identifying them 48 hours sooner than M-Enterococcus-vancomycin broth.

    Conclusions:

    • Enterococcel broth supplemented with 6 µg/mL vancomycin is a reliable method for the rapid and selective isolation of VRE.
    • This optimized broth formulation aids in timely VRE detection from surveillance specimens, supporting infection control strategies.