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Comparison of plasmid DNA preparation methods for direct gene transfer and genetic immunization
H L Davis1, M Schleef, P Moritz
1Loeb Medical Research Institute, Ottawa Civic Hospital, ON, Canada. hdavis@civich.ottawa.on.ca
Biotechniques
|July 1, 1996
Abstract:
Plasmid DNA is widely used for direct gene transfer in animals to study gene therapy, gene regulation, drug delivery and genetic immunization. Here we compare cesium chloride and anion-exchange purified plasmid DNA for direct gene transfer in mouse muscle and show no differences in efficiency of transfection with reporter genes or in humoral response to DNA-based immunization.