Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Functional interaction between DP-1 and p53

T S Sørensen1, R Girling, C W Lee

  • 1Laboratory of Eukaryotic Molecular Genetics, MRC National Institute for Medical Research, London, United Kingdom.

Molecular and Cellular Biology
|October 1, 1996
PubMed
Summary

The tumor suppressor p53 protein interacts with DP-1, a component of the DRTF1/E2F transcription factor. This interaction disrupts DP-1/E2F-1 DNA binding, suggesting a new way p53 controls cell proliferation.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

It's Time for Ireland's Tobacco-Free Future.

Irish medical journal·2026
Same author

Estimation of standardized ileal digestible calcium and phosphorus requirements of broiler chickens aged 21-35 days.

Poultry science·2025
Same author

Acute Respiratory Infection Outbreaks in Residential Care Facilities.

Irish medical journal·2025
Same author

Development and psychometric validation of the Chronic Rhinosinusitis Control Test.

Rhinology·2025
Same author

Correction: PPARγ is essential for protection against nonalcoholic steatohepatitis.

Gene therapy·2025
Same author

Efficacy and safety of home-based transcranial direct current stimulation as adjunct treatment for cognitive improvement in major depressive disorder: A double-blind, randomized, multi-site clinical trial.

European psychiatry : the journal of the Association of European Psychiatrists·2025

Area of Science:

  • Cellular biology
  • Molecular oncology
  • Transcription factor regulation

Background:

  • The transcription factor DRTF1/E2F regulates cell cycle genes, interacting with proteins like pRb.
  • The tumor suppressor p53 monitors DNA integrity and can halt cell cycle progression.
  • DP-1 and E2F-1 form DP/E2F heterodimers, mediating DRTF1/E2F DNA binding and transcriptional activity.

Purpose of the Study:

  • To investigate the potential association between DP-1 and p53.
  • To elucidate the functional consequences of this interaction on DRTF1/E2F activity and cell cycle control.

Main Methods:

  • Co-immunoprecipitation assays to detect protein-protein interactions in mammalian cell extracts.
  • In vitro binding assays to confirm direct interaction between p53 and DP-1.

Related Experiment Videos

  • Transcriptional assays to assess the impact of p53 on DP-1/E2F-1-driven transcription.
  • Mutational analysis to map interaction domains on DP-1 and p53.

    • Main Results:

    • An association between DP-1 and p53 was identified in mammalian cell extracts.
    • p53 directly interacts with DP-1 in vitro, competing with E2F-1 for binding.
    • This competition reduces DP-1/E2F-1 DNA binding activity and alters transcription.
    • Specific regions within DP-1 (C-terminal) and p53 (N-terminal) were identified as crucial for the interaction.

    Conclusions:

    • DRTF1/E2F is a common target for growth control pathways involving pRb and p53.
    • p53 can regulate cellular proliferation through an alternative mechanism involving direct interaction with DP-1.
    • This interaction provides a novel link between DNA damage response and cell cycle regulation via the DRTF1/E2F pathway.