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Related Experiment Videos

Intestinal Chlamydia in finishing pigs

L Szeredi1, I Schiller, T Sydler

  • 1Institute for Veterinary Pathology, Veterinary Medical Faculty, University of Zurich.

Veterinary Pathology
|July 1, 1996
PubMed
Summary
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Chlamydia trachomatis was detected in the large intestines of finishing pigs using immunohistochemistry. Enzyme-linked immunosorbent assay (ELISA) detected antibodies in most pigs, but agreement with other tests was poor.

Area of Science:

  • Veterinary Pathology
  • Microbiology
  • Immunology

Background:

  • Chlamydial infections are significant in livestock, impacting animal health and potentially zoonotic transmission.
  • Understanding Chlamydia prevalence and diagnostic accuracy in pigs is crucial for disease control.
  • Previous studies have focused on specific Chlamydia species, but a broader investigation in finishing pigs is needed.

Purpose of the Study:

  • To investigate the presence of Chlamydia in the gut and blood of finishing pigs.
  • To evaluate the diagnostic performance of immunohistochemistry (IHC) and serological tests (ELISA, CFT) for Chlamydia detection in pigs.
  • To determine the agreement between different diagnostic methods.

Main Methods:

  • Gut and blood samples were collected from 119 finishing pigs at slaughter.

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  • Immunohistochemistry (IHC) was performed on gut tissues using genus-specific and species-specific antibodies.
  • Antichlamydial antibodies in blood were detected using Enzyme-Linked Immunosorbent Assay (ELISA) and Complement Fixation Test (CFT).

    • Main Results:

    • Chlamydia inclusions were identified in the large intestine enterocytes of 29.7% of pigs by IHC.
    • ELISA detected antichlamydial antibodies in 82.6% of sera, while CFT was positive in 28.6%.
    • Agreement between ELISA and CFT was poor (kappa=0.112), but fair between IHC and CFT (kappa=0.205).

    Conclusions:

    • Chlamydia, particularly C. trachomatis, is present in the large intestine of finishing pigs.
    • ELISA is highly sensitive for detecting antichlamydial antibodies but shows poor agreement with CFT and IHC.
    • IHC is a valuable tool for identifying Chlamydia in pig tissues, with fair agreement with CFT for serological confirmation.