High performance thin layer chromatographic analysis of hydrolyzed tinidazole solutions. I. Development and...

A stability-indicating high performance thin layer chromatography method for analyzing hydrolyzed tinidazole solutions using silica gel plates was developed and validated. The mobile phase used was methanol-diethyl ether-chloroform (1:9:3, v/v/v) allowing small changes in its composition. Detection was at 314 mm. Rf values being 0.1-0.4, baseline resolution was achieved for tinidazole and the hydrolysis products. The analytes were stable on the sorbent and could be precisely and accurately measured in the range 20-170 ng per band.