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Published on: March 19, 2019
[Cyanide insensitive respiration of Candida lipolytica yeasts]
Abstract:
The effect of rotenone, antimycin A, oligomycin, 2,4,-DNP, and various subtrates on the appearance of cyanide-resistant respiration of Candida lipolytica yeast was studied. Rotenone inhibited the development of cyanide-resistant respiration, but did not prevent its origination. Antimycin A and oligomycin increased the rate of the appearance of cyanide-resistant respiration. If the resting yeast culture was incubated with 2,4-DNP, glucose, or glycerol, the development of the alternative pathway of electron transport was inhibited. Such substrates as alpha-glycerophosphate, citrate, and D,L-lactate had no effect on the time of appearance of cyanide-resistant respiration. Therefore, in the stationary growth phase, an alternative, cyanide-resistant pathway of electron transport results from the inhibition of the rate of electron transport in the respiration chain.
Insights
This study shows that specific inhibitors and substrates affect cyanide-resistant respiration in Candida lipolytica yeast. The alternative electron transport pathway develops due to inhibited respiration chain activity.
Area of Science:
- Biochemistry
- Microbiology
- Yeast Physiology
Background:
- Candida lipolytica exhibits cyanide-resistant respiration, an alternative electron transport pathway.
- Understanding the regulation of this pathway is crucial for yeast metabolism studies.
Purpose of the Study:
- To investigate the influence of various inhibitors and substrates on the emergence of cyanide-resistant respiration in Candida lipolytica.
- To elucidate the role of electron transport chain inhibition in the development of this alternative pathway.
Main Methods:
- Culturing Candida lipolytica yeast.
- Treating yeast cultures with specific inhibitors: rotenone, antimycin A, oligomycin, and 2,4-dinitrophenol (2,4-DNP).
- Incubating yeast with various substrates including glucose, glycerol, alpha-glycerophosphate, citrate, and D,L-lactate.
Main Results:
- Rotenone partially inhibited, but did not prevent, the development of cyanide-resistant respiration.
- Antimycin A and oligomycin accelerated the appearance of cyanide-resistant respiration.
- 2,4-DNP, glucose, and glycerol inhibited the development of the alternative pathway.
- Alpha-glycerophosphate, citrate, and D,L-lactate did not affect the timing of its appearance.
Conclusions:
- The development of cyanide-resistant respiration in stationary phase Candida lipolytica is linked to the inhibition of the main respiratory chain.
- Specific compounds can modulate the onset and activity of alternative electron transport pathways in yeast.
