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Updated: Aug 18, 2026

An Oligonucleotide-based Tandem RNA Isolation Procedure to Recover Eukaryotic mRNA-Protein Complexes
Published on: August 18, 2018
Micronuclear and macronuclear sequences of a Euplotes crassus gene encoding a putative nuclear protein kinase
C A Hale1, M E Jacobs, H G Estes
1Department of Biochemistry, University of Connecticut Health Center, Farmington 06030, USA.
Abstract:
The sequences of a 1.8-kbp macronuclear DNA molecule (V3), and the majority of its micronuclear counterpart, are reported. The macronuclear V3 DNA molecule contains an open reading frame that is interrupted by a single intron, while the micronuclear copy is interrupted by four internal eliminated sequences, one of which is located within the intron. The predicted protein product of the macronuclear V3 gene is a 471-amino acid polypeptide that is very similar to a group of protein-serine/threonine kinases from both plant and animal species, some of whose members appear to be involved in cell cycle or growth control.
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