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Anti-Di(b) as a red cell autoantibody
P D Issitt1, M R Combs, J Allen
1Transfusion Service, Duke University Medical Center, Durham, North Carolina, USA.
Transfusion
|September 1, 1996
Summary
Autoantibodies targeting the red cell anion exchanger protein band 3 were studied. While autoanti-Dib was found in 2.7% of samples, it was not the sole autoantibody present, unlike autoanti-Wrb.
Area of Science:
- Immunology
- Hematology
- Biochemistry
Background:
- Warm-reactive autoantibodies often target protein band 3, a red cell anion exchanger.
- Specific epitopes on band 3 include Wrb, Dia, and Dib, which result from amino acid polymorphism.
Purpose of the Study:
- To investigate the presence and specificity of autoantibodies against the Dib epitope on red blood cells.
- To compare the incidence of autoanti-Dib with autoanti-Wrb.
Main Methods:
- Testing autoantibodies from 119 patients against Di(b-) red blood cells.
- Absorbing 74 of these autoantibodies with Di(b-) red blood cells to identify specific antibody components.
Main Results:
- All 119 autoantibodies reacted with Di(b-) red cells, indicating no exclusive anti-Dib antibodies initially.
- Two of 74 adsorbed autoantibodies (2.7%) contained an unadsorbed anti-Dib component.
Conclusions:
- Autoanti-Dib was not found as the sole autoantibody in any of the 119 samples.
- The low incidence of autoanti-Dib contrasts with the higher incidence of autoanti-Wrb, despite both targeting band 3 epitopes.