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Related Experiment Videos

Mouse annexin V chromosomal localization, cDNA sequence conservation, and molecular evolution

M I Rodriguez-Garcia1, C A Kozak, R O Morgan

  • 1Department of Biochemistry and Molecular Biology, University of Oviedo, Spain.

Genomics
|January 15, 1996
PubMed
Summary

Researchers cloned mouse annexin V (ANX5) and mapped its gene to chromosome 3. Sequence analysis revealed high conservation and unique features in ANX5, aiding evolutionary rate estimation.

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Area of Science:

  • Molecular Biology
  • Genomics
  • Evolutionary Biology

Background:

  • Annexins are a family of calcium-dependent phospholipid-binding proteins involved in various cellular processes.
  • Mouse annexin V (ANX5) is a specific member of this family with incompletely understood functional and evolutionary characteristics.

Purpose of the Study:

  • To clone and sequence the full-length cDNA of mouse annexin V (ANX5).
  • To determine the chromosomal location of the ANX5 gene.
  • To analyze the sequence conservation and evolutionary relationships of ANX5 across different species.

Main Methods:

  • cDNA cloning and sequencing
  • Chromosomal mapping
  • Bioinformatic analysis including phylogenetic analysis and homology testing

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Main Results:

  • The full-length cDNA for mouse ANX5 was obtained and sequenced, encoding a 319 amino acid protein.
  • The ANX5 gene was mapped to mouse chromosome 3, linked to the fibroblast growth factor 2 (basic) gene.
  • High sequence identity (92-96%) was observed between mouse ANX5 and ANX5 from other species, with notable conservation in internal repeat 3.
  • Phylogenetic analysis suggested that Torpedo marmorata annexin may belong to the ANX5 subfamily.
  • An evolutionary mutation rate of 1% amino acid replacements per 8 million years was estimated for ANX5.

Conclusions:

  • Mouse ANX5 shares significant sequence homology with orthologs in other species, indicating functional conservation.
  • Specific regions within ANX5, particularly internal repeat 3, may possess unique properties distinguishing it from other annexins.
  • The study provides insights into the evolutionary dynamics of the annexin V subfamily.