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Molecular cloning of a genomic DNA for enolase from Aspergillus oryzae
M Machida1, T V Gonzalez, L K Boon
1Department of Molecular Biology, National Institute of Bioscience and Human-Technology, Ibaraki, Japan.
Bioscience, Biotechnology, and Biochemistry
|January 1, 1996
Abstract:
We have isolated an enolase gene (enoA) from Aspergillus oryzae by heterologous hybridization using the corresponding Saccharomyces cerevisiae ENO2 gene as a probe. A 2.9-kb BglII-fragment contained the entire structural gene enoA including 5'- and 3'- flanking regions. The homology between A. oryzae enoA and S. cerevisiae ENO2 genes is 66.9% when introns are removed. Genomic Southern analysis indicated that there is only one enolase gene in A. oryzae.