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Site-specific Bacterial Chromosome Engineering: ΦC31 Integrase Mediated Cassette Exchange (IMCE)

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Published on: March 16, 2012

The staphylococcal insertion sequence IS257 is active

C Needham¹, W C Noble, K G Dyke

¹Department of Biochemistry, University of Oxford, United Kingdom.

|November 1, 1995

Summary

This summary is machine-generated.

The IS257 element in Staphylococcus aureus is highly active, facilitating recombination and co-integration of plasmids. This mobile genetic element plays a role in antibiotic resistance gene transfer within hospital settings.

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Area of Science:

Microbiology
Molecular Biology
Genetics

Background:

Hospital-acquired infections often involve antibiotic-resistant bacteria.
Staphylococcus aureus is a common cause of nosocomial infections.
Plasmids and insertion sequences (IS) are key mobile genetic elements involved in antibiotic resistance.

Purpose of the Study:

To investigate the role of the IS257 insertion sequence in plasmid dynamics.
To characterize the genetic mechanisms of antibiotic resistance transfer in Staphylococcus aureus.
To understand the mobility of IS257 in a clinical isolate.

Main Methods:

Plasmid isolation and characterization from a clinical Staphylococcus aureus strain.
Analysis of insertion sequence (IS) element distribution and activity.
Investigation of plasmid recombination and co-integration events.

Main Results:

The plasmid pJ3356, conferring mupirocin resistance, contains two copies of IS257.
IS257 mediated the recombination of an erythromycin resistance plasmid (pOX7-IS) into pJ3356.
Co-integration of pJ3356 and pOX7 involved IS257 duplication and adjacent pOX7 sequence.
IS257 demonstrated active mobility and involvement in complex genetic rearrangements.

Conclusions:

IS257 is an active mobile genetic element in Staphylococcus aureus.
IS257 facilitates the transfer and integration of antibiotic resistance genes via plasmids.
Understanding IS257 activity is crucial for combating antibiotic resistance in clinical settings.