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Related Experiment Videos

Plasminogen activator system: implications for mammary cell growth and involution

I Politis1

  • 1Department of Animal and Food Sciences, University of Vermont, Burlington 05405, USA.

Journal of Dairy Science
|June 1, 1996
PubMed
Summary

Bovine mammary gland involution involves increased plasminogen activator, which converts plasminogen to plasmin. Bovine somatotropin (bST) treatment inhibited this plasmin increase, suggesting bST

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Area of Science:

  • Extracellular Proteolysis
  • Lactation Biology
  • Cellular Signaling

Background:

  • Plasminogen activators (PAs) mediate tissue remodeling by converting plasminogen to plasmin.
  • The plasmin-plasminogen system is implicated in bovine mammary gland involution.
  • PAs also play a role in cell proliferation.

Purpose of the Study:

  • To investigate the role of the plasmin-plasminogen system in bovine mammary gland involution.
  • To examine the effect of bovine somatotropin (bST) on plasminogen activation during involution.
  • To explore the relationship between plasminogen activator expression and cell proliferation in mammary epithelial cells.

Main Methods:

  • Measuring plasmin and plasminogen activator concentrations in bovine milk and mammary tissue.

Related Experiment Videos

  • Treating cows with bST during involution.
  • Culturing bovine mammary epithelial and myoepithelial cells and stimulating them with insulin, IGF-I, and phorbol myristate acetate.

    • Main Results:

    • Increased plasmin and plasminogen activator levels were observed during gradual involution of bovine mammary glands.
    • bST treatment prevented the rise in plasmin during involution, indicating interference with plasminogen conversion.
    • Insulin and IGF-I stimulated urokinase plasminogen activator synthesis and epithelial cell proliferation.
    • Phorbol myristate acetate increased urokinase plasminogen activator expression and myoepithelial cell proliferation, but not epithelial cell proliferation.

    Conclusions:

    • The plasmin-plasminogen system is associated with bovine mammary gland involution.
    • bST influences plasminogen to plasmin conversion during involution.
    • Plasminogen activator expression in bovine mammary cells is linked to proliferation but serves multiple functions beyond mitogenesis.