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Acidic and basic fibroblast growth factors prolong the in-vitro survival of murine peritoneal macrophages
1Department of Internal Medicine, Ohio State University, Columbus 43210, USA.
Abstract:
To examine the role of fibroblast growth factors on the proliferation present during angiogenic processes, we analyzed the effects of in vitro cell culture with acidic (FGF-1) and basic (FGF-2) fibroblast growth factors on murine peritoneal exudate cell survival. FGF-1 or FGF-2 in the presence of heparin resulted in a significantly greater cell viability for exudate cells at 24 hours of culture relative to heparin alone or medium controls. Cultures supplemented with FGF-1 or FGF-2 plus heparin showed a slight increase in tritiated[3H] thymidine uptake over heparin or medium alone. No specific change in [3H] uridine incorporation or LDH release was detectable between FGF-1, FGF-2, heparin or medium alone cultures. Our results suggest that FGF-1 and FGF-2 may enhance survival both in vitro and in vivo of a subset of peritoneal exudate cells. This attribute may play a role during inflammatory or tumor-like states.
Insights
Acidic (FGF-1) and basic (FGF-2) fibroblast growth factors enhance survival of peritoneal exudate cells in vitro. These findings suggest a role for FGF-1 and FGF-2 in inflammatory and tumor-like conditions.
Area of Science:
- Cell Biology
- Molecular Biology
- Immunology
Background:
- Fibroblast growth factors (FGFs) are crucial signaling molecules involved in various biological processes, including cell proliferation, differentiation, and survival.
- Angiogenesis, the formation of new blood vessels, is a complex process regulated by growth factors and is implicated in both physiological and pathological conditions such as inflammation and cancer.
- Peritoneal exudate cells (PECs) are a heterogeneous population of immune cells residing in the peritoneal cavity, playing a role in host defense and inflammatory responses.
Purpose of the Study:
- To investigate the effects of acidic fibroblast growth factor (FGF-1) and basic fibroblast growth factor (FGF-2) on the survival of murine peritoneal exudate cells (PECs) in vitro.
- To determine if FGF-1 and FGF-2 influence cellular proliferation and viability during conditions relevant to angiogenic processes.
Main Methods:
- Murine PECs were cultured in vitro with varying concentrations of FGF-1 or FGF-2, in the presence or absence of heparin.
- Cell viability was assessed at 24 hours using cell counting and viability assays.
- Cell proliferation was evaluated by measuring tritiated [3H] thymidine incorporation.
- Cellular damage was assessed by quantifying lactate dehydrogenase (LDH) release and [3H] uridine incorporation.
Main Results:
- Supplementation with FGF-1 or FGF-2, in combination with heparin, significantly increased PEC viability compared to heparin alone or medium controls at 24 hours.
- A slight increase in [3H] thymidine uptake was observed in cultures treated with FGF-1 or FGF-2 plus heparin, indicating a modest proliferative effect.
- No significant changes in [3H] uridine incorporation or LDH release were detected, suggesting that FGFs did not induce significant DNA/RNA synthesis changes or cell lysis under these conditions.
Conclusions:
- FGF-1 and FGF-2, particularly when combined with heparin, promote the survival of a subset of peritoneal exudate cells in vitro.
- These findings suggest that fibroblast growth factors may play a protective role for PECs, potentially influencing inflammatory responses and tumor microenvironments.
- The observed enhancement of cell survival by FGFs warrants further investigation into their in vivo functions in pathological states.
