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Related Experiment Videos

Expression of adhesion molecules on circulating PMN during hyperdynamic endotoxemia

K Holzer1, M Thiel, S Moritz

  • 1Institute for Surgical Research, Klinikum Grosshadern, Ludwig-Maximilians-University, Munich, Germany.

Journal of Applied Physiology (Bethesda, Md. : 1985)
|July 1, 1996
PubMed
Summary

Endotoxemia rapidly decreases L-selectin on polymorphonuclear leukocytes (PMN) while increasing their adhesion. Beta 2-integrin expression and function are dissociated, suggesting L-selectin shedding impedes PMN adhesion during endotoxemia.

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Area of Science:

  • Immunology
  • Cell Biology
  • Physiology

Background:

  • Endotoxemia involves complex inflammatory responses affecting leukocyte function.
  • Polymorphonuclear leukocytes (PMN) play a critical role in the inflammatory cascade.
  • L-selectin and beta 2-integrins are key adhesion molecules on PMN.

Purpose of the Study:

  • To investigate the expression and function of L-selectin and beta 2-integrins on PMN during hyperdynamic endotoxemia in a porcine model.
  • To determine the relationship between numerical expression and functional activity of beta 2-integrins.

Main Methods:

  • A porcine model of hyperdynamic endotoxemia induced by Salmonella abortus-equi endotoxin infusion.
  • Quantification of L-selectin and beta 2-integrin expression on circulating PMN using flow cytometry.

Related Experiment Videos

  • Assessment of PMN functional activation via adhesion to C3-coated zymosan particles.

    • Main Results:

    • Endotoxemia caused a rapid decrease in L-selectin expression on PMN.
    • PMN adhesion to C3-coated zymosan particles significantly increased, indicating functional upregulation of beta 2-integrins.
    • Numerical expression of beta 2-integrins remained unchanged initially but increased later in endotoxemia.
    • A dissociation was observed between the numerical and functional expression of beta 2-integrins.

    Conclusions:

    • The study demonstrates a dissociation between the numerical and functional expression of beta 2-integrins during endotoxemia.
    • Rapid L-selectin shedding and functional beta 2-integrin upregulation may contribute to PMN activation and altered adhesion.
    • Low L-selectin expression might hinder PMN adhesion despite beta 2-integrin activation.