Sensitive and fast mutation detection by solid phase chemical cleavage
Related Experiment Video
Updated: Oct 2, 2026

Wild-type Blocking PCR Combined with Direct Sequencing as a Highly Sensitive Method for Detection of Low-Frequency Somatic Mutations
Published on: March 29, 2017
L L Hansen1, J Justesen, T A Kruse
1Department of Human Genetics, Aarhus University, Denmark.
Abstract:
We have developed a solid phase chemical cleavage method (SpCCM) for screening large DNA fragments for mutations. All reactions can be carried out in microtiterwells from the first amplification of the patient (or test) DNA through the search for mutations. The reaction time is significantly reduced compared to the conventional chemical cleavage method (CCM), and even by using a uniformly labelled probe, the exact position and nature of the mutation can be revealed. The SpCCM is suitable for automatization using a workstation to carry out the reactions and a fluorescent detection-based DNA sequencing system to analyze the cleaved fragments.
Related Concept Videos
DNA Isolation
Restriction Enzymes
The host bacteria protect their own genomic DNA from these enzymes by methylating these sites. Some...
Maxam-Gilbert Sequencing
Challenges of the Maxam-Gilbert Method
The...

