Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Sorting by COP I-coated vesicles under interphase and mitotic conditions

B Sönnichsen1, R Watson, H Clausen

  • 1Cell Biology Laboratory, Imperial Cancer Research Fund, London, United Kingdom.

The Journal of Cell Biology
|September 1, 1996
PubMed
Summary

COP I vesicles maintain Golgi enzyme, cargo, and recycling protein levels during cell division. Their density variations suggest a role in Golgi transport, unaffected by cell cycle stage.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Unique Access to u-Channel Physics: Exclusive Backward-Angle Omega Meson Electroproduction.

Physical review letters·2019
Same author

Effects of nicotine exposure on murine mandibular development.

PloS one·2019
Same author

DHEA inhibits acute microglia-mediated inflammation through activation of the TrkA-Akt1/2-CREB-Jmjd3 pathway.

Molecular psychiatry·2017
Same author

SU-E-T-456: Potential Optimization of Stereotactic Body Radation Therapy (SBRT) from a Simple Field Timing Rearrangement.

Medical physics·2017
Same author

Nanogel-Conjugated Reverse Transcriptase Inhibitors and Their Combinations as Novel Antiviral Agents with Increased Efficacy against HIV-1 Infection.

Molecular pharmaceutics·2015
Same author

Amphiphilic cationic nanogels as brain-targeted carriers for activated nucleoside reverse transcriptase inhibitors.

Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology·2015

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • COP I vesicles mediate intra-Golgi transport and retrograde transport from the Golgi to the ER.
  • Understanding the protein composition and sorting mechanisms of COP I vesicles is crucial for elucidating Golgi apparatus function.

Purpose of the Study:

  • To analyze the protein content of COP I vesicles during different cell cycle stages (interphase vs. mitosis).
  • To determine the levels of resident Golgi enzymes, cargo proteins, and recycling proteins within COP I vesicles.
  • To investigate the functional implications of COP I vesicle composition and density in Golgi transport.

Main Methods:

  • Biochemical analysis of COP I vesicle content.
  • Morphological techniques to assess vesicle characteristics.

Related Experiment Videos

  • Quantification of resident Golgi enzymes, cargo, and recycling proteins.

    • Main Results:

    • Protein levels of resident Golgi enzymes (N-acetylgalactosaminyltransferase, N-acetylglucosaminyltransferase I, mannosidase II, galactosyltransferase), cargo (rat serum albumin, polyimmunoglobulin receptor), and recycling proteins (KDEL receptor, ERGIC-53/p58) were analyzed.
    • Vesicle protein content remained similar under interphase and mitotic conditions, indicating unaffected sorting.
    • Average densities relative to starting membranes were: resident enzymes (14-30%), cargo (16-23%), and recycling proteins (81-125%).

    Conclusions:

    • COP I vesicle sorting of resident enzymes, cargo, and recycling proteins is robust and unaffected by the cell cycle.
    • The distinct density profiles of different protein classes within COP I vesicles provide insights into their roles in Golgi transport.
    • These findings contribute to understanding the dynamic processes of protein trafficking within the Golgi apparatus.