Experiments in pigs on the sources of laser Doppler blood-flow signals recorded from teeth

A laser Doppler blood-flow meter (Moor Type MBF3D) was used to record from the crowns of eight deciduous mandibular incisors in three anaesthetized pigs. The flow-meter probe was attached to the crown either 2 or 6 mm from the gingival margin. Recordings were made from each tooth with the supporting tissues intact, after exposing the root pulp, after cutting the pulp, and after death of the animal. At each stage the effect of wrapping the crown in aluminium foil was determined. There was no significant change in the signals when the root pulp was exposed (p > 0.05, paired t-test). Without foil, cutting the pulp reduced the signal recorded 2 mm from the gingival margin by an average of 85.1 percent (n = 8, p < 0.001) and at 6 mm by 87.9 percent (n = 4, p < 0.001). There were further significant reductions (p < 0.01) equivalent to 12.9 percent and 9.0 percent, respectively, of the control values when the animal was killed. The foil increased the average blood-flow signals recorded from intact teeth at both 2 and 6 mm from the gingival margin by 77.8 percent and 98.3 percent (p < 0.05), respectively. It also had a similar effect after pulp exposure. With foil, cutting the pulp reduced the signal by 86.2 percent and 92.8 percent at the two sites, respectively, and post mortem there was a further reduction of 12.4 percent and 6.1 percent. Thus, under all conditions the major part of the signal recorded from an intact tooth was from the pulp but a significant component (an average of between 14.9 percent and 7.2 percent depending on the conditions) was from tissues outside the tooth. The best performance was obtained with foil and with the probe 6 mm from the gingival margin.