Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Endothelin peptides

Y Hirata1

  • 12nd Department of Internal Medicine, Tokyo Medical and Dental University, Japan.

Current Opinion in Nephrology and Hypertension
|January 1, 1996
PubMed
Summary
This summary is machine-generated.

Gene knockout studies reveal endothelin-1 (ET-1) and endothelin-3 (ET-3) are crucial for development. ET-1 signaling is vital for craniofacial and cardiovascular formation, while ET-3 is essential for neural crest development and pigmentation.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cellular mechanism of thrombin on endothelin-1 biosynthesis and release in bovine endothelial cell.

Biochemical pharmacology·1992
Same author

[A case of ALL with Candida liver abscess treated with amphotericin B through portal vein].

Nihon Naika Gakkai zasshi. The Journal of the Japanese Society of Internal Medicine·1992
Same author

A case of protracted suffocation death.

Nihon hoigaku zasshi = The Japanese journal of legal medicine·1992
Same author

Plasma endothelin-1 like immunoreactivity levels in neonates.

European journal of pediatrics·1992
Same author

Improvement in the heterogeneous N-termini and the defective N-glycosylation of human interleukin-6 by genetic engineering.

European journal of biochemistry·1992
Same author

Granulocyte-colony stimulating factor stimulates immunoreactive endothelin-1 release from cultured bovine endothelial cells.

European journal of pharmacology·1992
Same journal

Sex differences in renal acid-base regulation.

Current opinion in nephrology and hypertension·2026
Same journal

Primary prevention of chronic kidney disease in type 2 diabetes mellitus with sodium-glucose cotransporter 2 inhibitors.

Current opinion in nephrology and hypertension·2026
Same journal

Financial and policy challenges of delivering kidney replacement therapies in resource-limited settings.

Current opinion in nephrology and hypertension·2026
Same journal

The role of kir4.1/Kir5.1 in mediating the effect of angiotensin-II on Na-Cl-cotransporter.

Current opinion in nephrology and hypertension·2026
Same journal

Role of the calcium-sensing receptor in regulating calcium transport in the thick ascending limb.

Current opinion in nephrology and hypertension·2026
Same journal

Social determinants of chronic kidney disease: from association to clinical and population action.

Current opinion in nephrology and hypertension·2026
See all related articles

Area of Science:

  • Molecular Biology
  • Developmental Biology
  • Genetics

Background:

  • Endothelin isopeptides and their receptors play critical roles in physiological processes.
  • Gene knockout techniques provide powerful tools to elucidate gene function in vivo.

Purpose of the Study:

  • To investigate the developmental roles of endothelin-1 (ET-1) and endothelin-3 (ET-3) using gene knockout mouse models.
  • To characterize the endothelin-converting enzyme (ECE) and its role in ET-1 maturation.

Main Methods:

  • Generation and analysis of homozygous knockout mice for ET-1, ET-3, ETA, and ETB receptors.
  • Biochemical characterization of endothelin-converting enzyme.

Main Results:

  • Null mutations in ET-1 and ETA receptors resulted in craniofacial and cardiovascular malformations, highlighting their importance in first branchial arch development.

Related Experiment Videos

  • Null mutations in ET-3 and ETB receptors led to megacolon (Hirschsprung's disease) and coat spotting, indicating roles in neural crest-derived cell lineages, enteric neurons, and melanocytes.
  • Endothelin-converting enzyme was identified as a type II integral membrane metalloprotease homologous to neutral endopeptidase.

    • Conclusions:

    • Endothelin signaling pathways are essential for the proper development of multiple embryonic structures derived from neural crest cells.
    • Targeting endothelin-converting enzyme isoenzymes may offer therapeutic strategies for conditions related to endothelin dysregulation.