The PGK-PCR clonality assay (PPCA)
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Efficient Generation of hiPSC Neural Lineage Specific Knockin Reporters Using the CRISPR/Cas9 and Cas9 Double Nickase System
Published on: May 28, 2015
1Haematology and Immunology Centre, Hôpital Maisonneuve-Rosemont, Canada.
Molecular Biotechnology
|June 1, 1996
Abstract:
The PGK-PCR assay offers an accurate method for determination of clonal derivation of cells in females who are heterozygous for the PGK BstXI polymorphism. Techniques to screen for heterozygosity and determine clonality using polymerase chain reaction based techniques are provided.
