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Chimerism analysis after allogeneic bone marrow transplantation with nonradioactive RFLP and PCR-AFLP using the same
T Schreiner1, H Prochnow-Calzia, B Maccari
1Red Cross Blood Center, University of Ulm, Germany.
Journal of Immunological Methods
|September 13, 1996
Abstract:
We describe a method combining RFLP and PCR-AFLP analyses for studying chimerism after allogeneic bone marrow transplantation. Using RFLP analysis alone with DNA probe hMFl 87% of 244 donor/recipient pairs revealed different specific bands. By examination of the identical DNA probes using PCR-AFLP, an additional 52% of the residual donor/recipient pairs could be identified. Thus, 94% of allogeneic transplantations can be monitored using a combination of RFLP and PCR-AFLP analyses at one locus.