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Structural requirement of four-way junction DNA for binding to and cleavage by RuvC
T Shida1, H Iwasaki, H Shinagawa
1Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University, Nagano, Japan.
Nucleic Acids Symposium Series
|January 1, 1995
Abstract:
Sixteen bimobile four-way junction DNAs were designed to clarify the consensus sequence at the resolution site by the E. coli RuvC protein. The consensus sequence is 5'-T decreases X-3' (X: A, G, C, and T) without exception, but the sequences at the cleaved sites are not necessarily symmetric. The presence of symmetrically located two AT base-pairs at the junction is the minimal requirement for resolution.