Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Sequence-specific DNA recognition by peptide heterodimers

M Sawada1, Y Aizawa, M Ueno

  • 1Institute for Chemical Research, Kyoto University, Japan.

Nucleic Acids Symposium Series
|January 1, 1995
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Effects of phosphodiesterase inhibitors on cytokine production by microglia.

Multiple sclerosis (Houndmills, Basingstoke, England)·1999
Same author

Cosmetic osteoplastic craniotomy with a chisel and hammer.

Surgical neurology·1999
Same author

Clinicopathological study of chronic hepatitis induced by alcohol with or without hepatitis G virus.

Alcoholism, clinical and experimental research·1999
Same author

Effects of vesnarinone on cytokine production and activation of murine microglia.

Life sciences·1999
Same author

[Apoptosis and microglia].

Nihon yakurigaku zasshi. Folia pharmacologica Japonica·1999
Same author

Interleukin-10 inhibits both production of cytokines and expression of cytokine receptors in microglia.

Journal of neurochemistry·1999
Same journal

Nucleotide specificity of DNA cleavage by esperamycin/calichemicin antitumor antibiotics.

Nucleic acids symposium series·2018
Same journal

A model reaction for the repair of nucleic acids. The repair of thymidine bromohydrins.

Nucleic acids symposium series·2018
Same journal

Effects of base damages on DNA replication.

Nucleic acids symposium series·2018
Same journal

Effect of serine residue on the effectiveness of cationic polypeptide-based gene delivery.

Nucleic acids symposium series·2003
Same journal

Smart probe: a novel fluorescence quenching-based oligonucleotide probe carrying a fluorophore and an intercalator.

Nucleic acids symposium series·2003
Same journal

The engineering, structure, and DNA binding properties of a novel His4-type zinc finger peptide.

Nucleic acids symposium series·2003
See all related articles

Peptide heterodimers engineered from basic leucine zipper proteins achieve sequence-specific DNA recognition. This DNA binding depends on the stability and structural differences of matched versus half-matched complexes.

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Protein Engineering

Background:

  • Basic amino acid-rich DNA binding motifs are crucial for sequence-specific DNA recognition by proteins.
  • Basic leucine zipper (bZIP) proteins are a class of transcription factors that bind DNA as dimers.

Purpose of the Study:

  • To engineer peptide heterodimers capable of sequence-specific DNA recognition.
  • To investigate the role of an artificial dimerization module in controlling hetero-dimerization.
  • To analyze the influence of binding complex stability on sequence discrimination efficiency.

Main Methods:

  • Design and synthesis of peptide heterodimers derived from bZIP proteins.
  • Incorporation of a host-guest inclusion complex for controlled hetero-dimerization.

Related Experiment Videos

  • Analysis of DNA binding affinity and specificity for matched and half-matched DNA sequences.
  • Structural studies of DNA-peptide complexes.

    • Main Results:

    • Peptide heterodimers demonstrated sequence-specific DNA recognition.
    • An artificial dimerization module successfully controlled specific hetero-dimerization.
    • The efficiency of sequence discrimination was correlated with the stability of the half-matched binding complex.
    • Distinct structural differences were observed between matched and half-matched DNA-peptide binding complexes.

    Conclusions:

    • Engineered peptide heterodimers can achieve specific DNA sequence recognition.
    • Artificial dimerization modules offer a method for controlling protein-protein interactions in DNA binding.
    • The stability and structure of DNA-protein complexes are critical determinants of binding specificity.