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Mechanism of antisense effect studied by functionalized oligonucleotides
A Murakami1, A Yoshimoto, A Furumoto
1Department of Polymer Science and Engineering, Kyoto Institute of Technology, Japan.
Abstract:
In order to study the mechanism of antisense effects, various functional groups such as photocrosslinkers, fluorescent materials, and gold particles were introduced to antisense DNAs. Upon UV-irradiation, the hybrid between DNA and antisense DNA with a photocrosslinker was stabilized. The fluorescence microscopy and transmission electron microscopy (TEM) represented clear images of antisense DNA in the cultured cells. Fluorescent-labeled S-Oligos were recognized in the cytoplasm and especially on certain organs, which are not specified. TEM images of cultured cells treated with gold-conjugated S-Oligo suggested the mechanism of cellular uptake of antisense DNA.