Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

E-selectin expression on human brain microvascular endothelial cells

D C Hess1, Y Thompson, A Sprinkle

  • 1Neurology Service (127), V.A. Medical Center, Augusta, GA, USA.

Neuroscience Letters
|July 26, 1996
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Recurrent primary scrotal extramammary Paget disease: a case report and literature review.

Annals of the Royal College of Surgeons of England·2023
Same author

Effects of color-enhancing glasses on color vision in congenital red-green color deficiencies.

Optics express·2022
Same author

Theoretical versus empirical measures of retinal magnification for scaling AOSLO images.

Journal of the Optical Society of America. A, Optics, image science, and vision·2021
Same author

Erratum: Azimuthal Anisotropy of K_{S}^{0} and Λ+Λ[over ¯] Production at Midrapidity from Au+Au Collisions at sqrt[s]_{NN}=130  GeV [Phys. Rev. Lett. 89, 132301 (2002)].

Physical review letters·2021
Same author

Erratum: Azimuthal Anisotropy at the Relativistic Heavy Ion Collider: The First and Fourth Harmonics [Phys. Rev. Lett. 92, 062301 (2004)].

Physical review letters·2021
Same author

Measurement of the Positive Muon Anomalous Magnetic Moment to 0.46 ppm.

Physical review letters·2021
Same journal

Dynorphinergic neuroadaptations in the islands of Calleja: implications for alcohol use disorder.

Neuroscience letters·2026
Same journal

Differential vulnerability of cochlear nuclei to Lmx1 deficiency: abnormal patterning and implications for auditory circuitry.

Neuroscience letters·2026
Same journal

Role of nNOS/sGC pathway in the insular cortex in control of cardiovascular, autonomic and corticosterone responses to restraint stress in rats.

Neuroscience letters·2026
Same journal

Jak1 inhibition reduces acute allodynia induced by specific upstream cytokines in rats: implications for the onset of Jak1 pain modulation.

Neuroscience letters·2026
Same journal

Glucocorticoids-induced depressive-like behaviors in mice: oral ingestion of corticosterone or hydrocortisone - A comparative study.

Neuroscience letters·2026
Same journal

Data-driven clustering of prefrontal activation identifies functional phenotypes under prioritized dual-task walking conditions in Parkinson's disease.

Neuroscience letters·2026
See all related articles

E-selectin expression in brain endothelial cells is induced by inflammatory stimuli. N-acetylcysteine and dexamethasone inhibit this expression, suggesting a role in mitigating brain inflammation.

Area of Science:

  • Neuroscience
  • Immunology
  • Cell Biology

Background:

  • E-selectin is an endothelial adhesion molecule crucial for neutrophil binding and targeting.
  • Its expression and role within the brain microvasculature remain largely uncharacterized.

Purpose of the Study:

  • To investigate the expression of E-selectin on human brain microvascular endothelial cells (HBMEC).
  • To explore the modulatory effects of specific compounds on E-selectin expression and related inflammatory pathways in HBMEC.

Main Methods:

  • Cultured HBMEC were stimulated with inflammatory cytokines (IL-1b, TNF) and lipopolysaccharide (LPS).
  • E-selectin surface expression was quantified using flow cytometry.
  • The impact of N-acetylcysteine (NAC), dimethylthiourea (DMTU), dexamethasone, and allopurinol on E-selectin expression was assessed.

Related Experiment Videos

  • Nuclear factor kappa B (NF-κB) activation by TNF was measured in HBMEC.

    • Main Results:

    • Basal E-selectin expression was absent in HBMEC but induced by IL-1b, TNF, or LPS stimulation within 4 hours.
    • Induced E-selectin expression on HBMEC was quantitatively lower compared to human umbilical vein endothelial cells (HUVEC).
    • N-acetylcysteine (NAC), dimethylthiourea (DMTU), and dexamethasone partially inhibited cytokine-induced E-selectin upregulation.
    • TNF-induced NF-κB activation in HBMEC was attenuated by NAC and dexamethasone treatment.

    Conclusions:

    • E-selectin is expressed on brain microvascular endothelial cells upon inflammatory stimulation.
    • Thiol donors (like NAC) and corticosteroids (like dexamethasone) can inhibit E-selectin expression and NF-κB activation.
    • These findings suggest a potential therapeutic role for thiol donors and corticosteroids in managing neuroinflammatory conditions by modulating neutrophil-endothelial interactions.