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Extracellular matrix on intraocular lenses
S Saika1, A Yamanaka, S Tanaka
1Department of Ophthalmology, Wakayama Medical College, Japan.
Experimental Eye Research
|December 1, 1995
Summary
Extracellular matrix components like fibronectin, collagen, and vitronectin were found on explanted intraocular lenses (IOLs). These deposits suggest poor granulation tissue formation, potentially impacting IOL biocompatibility.
Area of Science:
- Ophthalmology
- Biomaterials Science
- Cell Biology
Background:
- Intraocular lenses (IOLs) are implanted during cataract surgery.
- Proteinaceous and cellular deposits can form on explanted IOLs.
- Understanding these deposits is crucial for assessing IOL biocompatibility.
Purpose of the Study:
- To investigate the composition of extracellular matrix (ECM) components within the proteinaceous deposits on explanted IOLs.
- To clarify the nature and origin of these deposits on IOL surfaces.
Main Methods:
- Immunohistochemistry was used to examine explanted IOLs.
- Fifteen polymethylmethacrylate (PMMA) IOLs and one silicone IOL were analyzed.
- Staining was performed for cellular fibronectin, types I and IV collagen, and vitronectin.
Main Results:
- Fibronectin, types I and IV collagen, and vitronectin were detected in the ECM of all explanted IOLs with cellular deposits.
- Types I and IV collagen and cellular fibronectin were likely produced by adhered cells.
- Vitronectin appeared to be adsorbed from the aqueous humor.
Conclusions:
- The presence of these ECM components indicates an unsuccessful granulation tissue formation.
- These proteinaceous and cellular deposits may negatively influence IOL biocompatibility.
- Further research is needed to fully understand the implications for long-term IOL performance.