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Related Experiment Videos

Poly(L-lactic acid) foams with cell seeding and controlled-release capacity

H Lo1, S Kadiyala, S E Guggino

  • 1Department of Chemical Engineering, Johns Hopkins University, Baltimore, Maryland, USA.

Journal of Biomedical Materials Research
|April 1, 1996
PubMed
Summary

Researchers developed poly(l-lactic acid) (PLLA) foams for tissue engineering. These porous scaffolds support cell growth and enable sustained release of nutrients, mimicking natural tissue structures for regenerative medicine applications.

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Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Polymer Chemistry

Background:

  • Ideal tissue engineering scaffolds require porous, 3D structures that mimic native tissues.
  • Such scaffolds should facilitate nutrient delivery and cell integration for regenerative purposes.
  • Poly(l-lactic acid) (PLLA) is a biocompatible polymer suitable for biomedical applications.

Purpose of the Study:

  • To fabricate and characterize poly(l-lactic acid) (PLLA) foams as potential tissue engineering scaffolds.
  • To evaluate the capacity of PLLA foams for sustained release of incorporated substances.
  • To assess the performance of PLLA foams as a cell culture substrate for bone cells.

Main Methods:

  • Fabrication of PLLA foams using phase separation from naphthalene solutions.

Related Experiment Videos

  • Characterization of foam properties including density, pore-surface area, and loss tangent.
  • Assessment of naphthalene residue removal via vacuum sublimation.
  • Incorporation and release studies using model compounds (bromothymol blue, sulforhodamine B) and alkaline phosphatase.
  • Cell culture studies using rat osteosarcoma cells (ROS 17/2.8) and UMR-106 cells.

    • Main Results:

    • PLLA foams with uniform, open cells were successfully fabricated with densities of 0.05-0.1 g/cm³ and pore-surface areas of 0.8-1.3 m²/g.
    • Naphthalene residue was reduced to below 0.2 wt% after vacuum sublimation.
    • Sustained release of bromothymol blue was observed for over 2 months from foams with 87% porosity.
    • Model protein alkaline phosphatase retained approximately 70% of its bioactivity after incorporation.
    • PLLA foams supported cell growth and function comparable to flat PLLA surfaces, with UMR-106 cells showing enhanced mineralization.

    Conclusions:

    • PLLA foams fabricated via phase separation are promising candidates for tissue engineering scaffolds.
    • These foams offer controlled release of incorporated substances and support cell growth and function.
    • The porous PLLA structure enhances mineralization in certain cell types, indicating potential for bone tissue regeneration.