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Related Experiment Videos

Determination of cyanide using a microbial sensor

K Nakanishi1, K Ikebukuro, I Karube

  • 1Research Center for Advanced Science and Technology, University of Tokyo, Japan.

Applied Biochemistry and Biotechnology
|August 1, 1996
PubMed
Summary

This study presents a novel microbial cyanide sensor using Saccharomyces cerevisiae and an oxygen electrode. The sensor effectively measures cyanide concentrations by monitoring changes in microbial respiration rates.

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Area of Science:

  • Biotechnology
  • Environmental Science
  • Biomedical Engineering

Background:

  • Cyanide is a highly toxic pollutant requiring sensitive detection methods.
  • Existing cyanide detection methods can be complex or lack sensitivity.
  • Microbial biosensors offer a promising alternative for environmental monitoring.

Purpose of the Study:

  • To develop and characterize a microbial biosensor for cyanide detection.
  • To evaluate the sensor's performance in a controlled batch system.
  • To establish the linear range and sensitivity of the cyanide sensor.

Main Methods:

  • Immobilization of Saccharomyces cerevisiae onto an oxygen electrode.
  • Monitoring changes in microbial respiration in response to glucose.

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  • Measuring the inhibitory effect of cyanide on mitochondrial electron transport.
  • Utilizing an oxygen electrode to quantify respiration rate changes.

    • Main Results:

    • The microbial sensor demonstrated a clear correlation between cyanide concentration and respiration inhibition.
    • A linear calibration curve was observed for cyanide concentrations ranging from 0.3 microM to 150 microM.
    • The sensor operated effectively at pH 8.0 and 30 degrees C.

    Conclusions:

    • The developed Saccharomyces cerevisiae-based biosensor is a viable tool for sensitive cyanide detection.
    • The sensor offers a cost-effective and efficient method for monitoring cyanide levels.
    • This technology has potential applications in environmental and industrial safety monitoring.