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Spontaneous degranulation of cultured bone-marrow-derived immature mast cells from X-linked immunodeficient (Xid)
A M Dvorak1, T Miura, L Letourneau
1Department of Pathology, Beth Israel Hospital, Boston, MA 02215, USA.
IgE-mediated, antigen-dependent stimulation of immature mouse mast cells cultured in IL-3-containing media produces secretion by granule exocytosis. Similar cultured mast cells were derived from X-linked immunodeficient (Xid) mice and examined by electron microscopy. In these cultures, Xid mast cells were also immature. In contrast to cultures obtained from control mice, 10-20% of the immature mast cells of Xid origin were undergoing secretion by granule extrusion in the absence of any secretogogue. Spontaneous secretion may be related to discordered tyrosine kinase function and/or signal transduction pathways in the Xid mouse.
IgE-mediated, antigen-dependent stimulation of immature mouse mast cells cultured in IL-3-containing media produces secretion by granule exocytosis. Similar cultured mast cells were derived from X-linked immunodeficient (Xid) mice and examined by electron microscopy. In these cultures, Xid mast cells were also immature. In contrast to cultures obtained from control mice, 10-20% of the immature mast cells of Xid origin were undergoing secretion by granule extrusion in the absence of any secretogogue. Spontaneous secretion may be related to discordered tyrosine kinase function and/or signal transduction pathways in the Xid mouse.