Rapid GLC determination of therapeutic plasma glycerin levels

A new rapid and inexpensive method for the determination of therapeutic plasma glycerin concentrations is described. In this method, acetic anhydride and pyridine are added to 15 microliter of plasma. After brief incubation and centrifugation, an aliquot of the supernate is injected directly onto the 3% OV-1 column. A linear calibration curve was found in the 0.05--3-mg/ml range, with the precision of the assay estimated to be +/-5.5% (RSD). The method was used to determining preliminary pharmacokinetic data in the rabbit.