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Related Experiment Videos

Putative intestinal estrogen receptor: evidence for regional differences

M A Salih1, S H Sims, D N Kalu

  • 1Department of Physiology, University of Texas Health Science Center, San Antonio 78284-7756, USA.

Molecular and Cellular Endocrinology
|July 23, 1996
PubMed
Summary

Estrogen receptors (ERs) are present in rat intestinal cells, regulating calcium absorption. The duodenum shows a variant ER gene, suggesting a unique role in intestinal estrogen signaling.

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Area of Science:

  • Endocrinology
  • Molecular Biology
  • Gastroenterology

Background:

  • 17 beta-estradiol (E2) stimulates intestinal calcium absorption and uptake by mucosal cells.
  • Intestinal mucosal cells contain estrogen receptor (ER)-like proteins and mRNAs.

Purpose of the Study:

  • To characterize the putative intestinal ERs.
  • To investigate the presence and nature of ERs in different segments of the rat intestine.

Main Methods:

  • RT-PCR analysis
  • Western blot analysis
  • Southern blot analysis
  • Ligand binding assays
  • Gel shift assays

Main Results:

  • ER mRNAs were found throughout the intestine, except in the duodenum.

Related Experiment Videos

  • Western blot detected ER proteins of various sizes, with duodenal extracts showing distinct bands.
  • Ligand binding assays confirmed E2 binding to a 46-kDa protein in duodenum, colon, and uterus.
  • Gel mobility shift assays indicated the presence of a classical DNA binding domain in jejunal, colonic, and uterine ERs, but not in duodenal extracts.

    • Conclusions:

    • ER-like proteins and genes are present in rat intestinal mucosal cells.
    • The duodenum appears to contain a variant ER gene encoding a variant ER protein, distinct from those in other intestinal segments.