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Related Experiment Videos

A novel physiological function for platelet-derived growth factor-BB in rat dermis

S A Rodt¹, K Ahlén, A Berg

¹Department of Medical and Physiological Chemistry, University of Uppsala, Sweden.

The Journal of Physiology

|August 15, 1996

Summary

Platelet-derived growth factor-BB (PDGF-BB) and alpha 2 beta 1-integrin regulate interstitial fluid pressure (Pif). Blocking alpha 2 beta 1-integrin lowers Pif, but PDGF-BB administration normalizes it, revealing a novel role for PDGF-BB in Pif control.

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Area of Science:

Physiology
Cell Biology
Biochemistry

Background:

Interstitial fluid pressure (Pif) is a critical physiological parameter.
The regulation of Pif by cellular adhesion receptors and growth factors is not fully understood.

Purpose of the Study:

To investigate the role of platelet-derived growth factor-BB (PDGF-BB) and alpha 2 beta 1-integrin in controlling rat skin interstitial fluid pressure (Pif).

Main Methods:

Interstitial fluid pressure (Pif) was measured in rat skin using glass capillaries and a servocontrolled counter-pressure system.
Subdermal injections of anti-alpha 2 beta 1-integrin antibodies and PDGF-BB were administered.
Dextran anaphylaxis was used as a model to induce changes in Pif.

Main Results:

Related Experiment Videos

Blocking alpha 2 beta 1-integrin with antibodies significantly decreased Pif, making it more negative.
Co-administration of PDGF-BB with anti-alpha 2 beta 1-integrin antibodies abolished the effect of the antibodies.
PDGF-BB administration normalized Pif during dextran-induced anaphylaxis, while PDGF-AA and basic fibroblast growth factor had no effect.

Conclusions:

Alpha 2 beta 1-integrin plays a role in controlling interstitial fluid pressure (Pif).
Platelet-derived growth factor-BB (PDGF-BB) can counteract the effects of alpha 2 beta 1-integrin blockade on Pif.
These findings suggest PDGF-BB actively participates in regulating Pif by modulating connective tissue cell interactions with the extracellular matrix.