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The determination of the extracellular concentration of brain glutamate using quantitative microdialysis
M Miele1, M Berners, M G Boutelle
1University Laboratory of Physiology, Oxford, UK.
Brain Research
|January 22, 1996
Abstract:
Quantitative microdialysis with two enzyme-based assays was used to determine the extracellular concentration of glutamate in the striatum of freely moving rats. From the difference between infused and dialysate glutamate a value of 3.0 +/- 0.6 microM for the extracellular glutamate concentration was computed by regression analysis. The in vivo recovery, derived from the slope of the regression line, was 50%.