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Updated: Aug 8, 2026

Application of Electrophysiology Measurement to Study the Activity of Electro-Neutral Transporters
Published on: February 3, 2018
[Distribution of certain nonelectrolytes between intra- and extracellular parts of erythrocytes]
Abstract:
Human red blood cells were balanced at 37 degrees C in 15% solution of the following nonelectrolytes (NE): methanol, ethanol, 1,2-propanediol, diethylene glycol, glycerol, polythelene glycols (PEGs) with molecular masses 300, 400, 600, 1000, 15000. The distribution coefficients (Q) equal to the ratio of the N concentration in cell to that in the intercellular medium, were determined by 1H-NMR technique. A monotonous decrease of the Q values with the increase of the molecular mass of the NE from 1.15 + 0.12 for methanol to 0.12 +/- 0.01 for PEG-1500 has been demonstrated. Results obtained demonstrate that the mixture (PEG) components with different molecular weights and 1,2-PD-PEG-1500 take negligible influence on the distribution coefficients of these substances between erythrocytes and extracellular environment.
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