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Related Experiment Videos

Changes in HL-60 cell deformability during differentiation induced by DMSO

M A Tsai1, R E Waugh, P C Keng

  • 1Department of Biophysics, University of Rochester School of Medicine and Dentistry, NY 14642, USA. mts1@medinfo.rochester.edu

Biorheology
|January 1, 1996
PubMed
Summary

Cellular deformability increases as promyelocytic leukemic HL-60 cells mature, with cytoplasmic viscosity significantly decreasing. This study quantifies changes in cell mechanics during differentiation.

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Area of Science:

  • Biophysics
  • Cell Biology
  • Hematology

Background:

  • Promyelocytic leukemic HL-60 cells are a model for studying cell differentiation.
  • Cellular mechanics, including deformability and viscosity, change during cell maturation.
  • Understanding these changes is crucial for hematological research.

Purpose of the Study:

  • To investigate alterations in cellular deformability and cytoplasmic viscosity during HL-60 cell maturation.
  • To quantify the relationship between cell maturation and biophysical properties.

Main Methods:

  • HL-60 cells were induced to differentiate using dimethyl sulfoxide.
  • Single-cell micropipette aspiration was employed to measure cellular deformability.
  • Cytoplasmic viscosity was calculated and analyzed for power-law dependence on shear rate.

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Main Results:

  • Cellular size decreased, while deformability increased with HL-60 cell maturation.
  • Cytoplasmic viscosity decreased significantly from 210-85 Pa.s (pre-induction) to 85-40 Pa.s (Day 7).
  • Cytoplasmic viscosity showed a power-law dependence on shear rate (b ≈ 0.5), with characteristic viscosity decreasing upon maturation.

Conclusions:

  • HL-60 cell maturation is associated with decreased size and increased deformability.
  • Cytoplasmic viscosity reduces during differentiation, indicating significant changes in cell mechanics.
  • The findings provide quantitative insights into the biophysical transformations during leukemic cell maturation.