Distinct composition of viral quasispecies between ascites and serum samples from patients with late stage chronic...

By performing single-strand conformation polymorphism and PCR-cloning followed by sequencing, the amino acid composition of hepatitis C virus hypervariable and variable regions were analyzed in ascites and serum samples from two patients with late stage chronic hepatitis C. In patient-LC, the major ascitic clone was constituted by a minor serum-derived clone, while the major serum derived clone was absent in the ascites. In patient-HCC, the major ascitic clone was defective and genetically different from all the serum-derived clones. In addition, anti-genomic HCV RNA was detected in the ascitic mononuclear cells from patient-LC. These results argued that the peritoneal cavity served as a relatively isolated environment for evolution of distinct composition of viral quasispecies.