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Cytogenetic effects in mice of divinylbenzene-55 inhalation
A D Kligerman1, D L Morgan, C L Doerr
1Environmental Carcinogenesis Division, U.S. Environmental Protection Agency, Research Triangle Park, NC 27711, USA. kligerman@her145.Her1.Epa.Gov
Abstract:
Male B6C3F1 mice (8 weeks of age) were exposed by inhalation to divinylbenzene-55 (DVB-55), at target concentrations of 0, 25, 50 and 75 ppm for 6 h per day for 3 days. Following exposure the animals were killed blood smears were prepared for micronucleus (MN) analysis, and the spleens were removed and cultured for sister chromatid exchange (SCE) and chromosome aberration (CA) analyses. DVB-55 induced a dose dependent increase in SCE with the two highest doses reaching statistical significance. Similarly, there was a statistically significant although less pronounced increase in the frequency of CAs in splenocytes and MN in polychromatic erythrocytes. There was no indication of toxicity as measured by cell cycle kinetics in the splenocytes or the percentage of polychromatic erythrocytes in the peripheral blood smears. Thus, DVB-55 appears to be a weak genotoxicant in vivo.
Insights
Divinylbenzene-55 (DVB-55) exposure in mice showed a dose-dependent increase in genetic damage, including sister chromatid exchange and chromosome aberrations. DVB-55 was identified as a weak genotoxicant in vivo.
Area of Science:
- Toxicology
- Genetics
Background:
- Divinylbenzene-55 (DVB-55) is an industrial chemical with potential health implications.
- Assessing the genotoxicity of DVB-55 is crucial for understanding its in vivo effects.
Purpose of the Study:
- To evaluate the genotoxic potential of DVB-55 in male B6C3F1 mice following inhalation exposure.
- To investigate DVB-55's effects on chromosomal damage and genetic mutations.
Main Methods:
- Mice were exposed to DVB-55 at concentrations of 0, 25, 50, and 75 ppm for 6 hours daily over 3 days.
- Micronucleus (MN) analysis, sister chromatid exchange (SCE), and chromosome aberration (CA) assays were performed on collected samples.
- Cell cycle kinetics and erythrocyte counts were assessed to evaluate toxicity.
Main Results:
- DVB-55 exposure led to a dose-dependent increase in SCE, with significant results at higher concentrations.
- A statistically significant, though less pronounced, increase in CA and MN frequencies was observed.
- No signs of toxicity were detected based on cell cycle kinetics or erythrocyte parameters.
Conclusions:
- DVB-55 demonstrates weak genotoxic activity in vivo.
- The study suggests DVB-55 can induce genetic damage at the cellular level without causing overt toxicity.