Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Requirements for perpendicular helix pairing

J R Beasley1, G J Pielak

  • 1Department of Chemistry, University of North Carolina at Chapel Hill 27599-3290, USA.

Proteins
|September 1, 1996
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Solvent-induced collapse of alpha-synuclein and acid-denatured cytochrome c.

Protein science : a publication of the Protein Society·2001
Same author

Characterization of horse cytochrome c expressed in Escherichia coli.

Protein expression and purification·2001
Same author

Interpreting the effects of small uncharged solutes on protein-folding equilibria.

Annual review of biophysics and biomolecular structure·2001
Same author

Second virial coefficients as a measure of protein--osmolyte interactions.

Protein science : a publication of the Protein Society·2001
Same author

Interactions between yeast iso-1-cytochrome c and its peroxidase.

Biochemistry·2001
Same author

Effects of crowding by mono-, di-, and tetrasaccharides on cytochrome c-cytochrome c peroxidase binding: comparing experiment to theory.

Biochemistry·2001
Same journal

Engineered HSP90-MP65 Bivalent Fusion Antigen: A Novel Vaccine Candidate Against Invasive Candidiasis.

Proteins·2026
Same journal

Physics-Based Energy Functions for Computational Protein Design.

Proteins·2026
Same journal

Impact of Stabilizing Osmolytes on the Conformational Dynamics of Human and Rat Islet Amyloid Polypeptides.

Proteins·2026
Same journal

Stabilization of Bone Morphogenetic Protein-2 at Physiological pH: Contrasting Roles of CHAPS and Arginine in Aggregation Inhibition.

Proteins·2026
Same journal

Structural Insights Into the Function of Leishmania major Adenylosuccinate Lyase.

Proteins·2026
Same journal

Generalizing the Gaussian Network Model: Spanning-Tree Thermodynamics Shows Entropy-Driven KRAS Activation.

Proteins·2026
See all related articles

Cassette mutagenesis created a yeast iso-1-cytochrome c mutant library. Functional cytochrome c variants showed correlations with mutation matrices and binary amino acid patterns.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Genetics

Background:

  • Cytochrome c is crucial for cellular respiration.
  • Understanding protein structure-function relationships is key in molecular biology.
  • Targeting specific protein interfaces can reveal functional constraints.

Purpose of the Study:

  • To generate and analyze mutations at the N- and C-terminal helices interface of Saccharomyces cerevisiae iso-1-cytochrome c.
  • To identify amino acid substitutions that maintain cytochrome c function.
  • To correlate mutation phenotypes with biophysical properties and sequence patterns.

Main Methods:

  • Cassette mutagenesis to create a random mutation library.
  • Functional screening of over 11,000 yeast mutants based on growth on nonfermentable carbon sources.

Related Experiment Videos

  • Analysis of genotype-phenotype correlations using the Dayhoff mutation matrix and transfer free energies.

    • Main Results:

    • Approximately 0.5% of possible amino acid combinations at four key residues yielded functional cytochrome c.
    • Significant correlations were observed between yeast growth phenotypes and mutation matrices/transfer free energies.
    • Functional sequences exhibited a discernible binary amino acid pattern.

    Conclusions:

    • The study successfully mapped functional constraints at the cytochrome c interface.
    • Protein sequence and biophysical properties are critical determinants of cytochrome c function.
    • A binary amino acid pattern underlies functional cytochrome c variants at this interface.