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[Persistent infection in murine L cells infected with the parainfluenza virus SV5]
Abstract:
Persistent infection of mouse L cells was achieved by serial passages of undiluted parainfluenza SV5 virus. At the level of the 10--12th passage a stationary noncytocidal type of persistent infection was established. A low level of the infectious virus produced by the persistently infected cells was observed; the number of cells synthesizing the virus-specific antigen detectable by immunofluorescence was high. Persistently infected cells were resistant to the infection with the homologous virus and sensitive to superinfection with heterologous viruses. The persisting virus had marked interfering activity. The possibility of participation of defective interfering particles and endogenous oncornavirus in the maintenance of the persistent infection is discussed.
Insights
Researchers established a persistent parainfluenza virus (SV5) infection in mouse cells, creating a stable state where cells produced low infectious virus levels but high viral antigens. These infected cells showed resistance to SV5 re-infection.
Area of Science:
- Virology
- Cell Biology
- Immunology
Background:
- Persistent viral infections can alter host cell behavior and immune responses.
- Understanding the mechanisms of persistent paramyxovirus infections is crucial for developing therapeutic strategies.
Purpose of the Study:
- To establish and characterize a persistent infection model using parainfluenza virus SV5 in mouse L cells.
- To investigate the characteristics of virus production, antigen expression, and cellular response in persistently infected cells.
Main Methods:
- Serial passage of undiluted parainfluenza virus SV5 in mouse L cells.
- Assessment of infectious virus production and viral antigen synthesis via immunofluorescence.
- Evaluation of cellular resistance and sensitivity to homologous and heterologous virus superinfection.
Main Results:
- A stable, non-cytocidal persistent infection was established by the 10th-12th passage.
- Persistently infected cells produced low levels of infectious virus but high levels of virus-specific antigens.
- Infected cells exhibited resistance to homologous SV5 superinfection and sensitivity to heterologous viruses, indicating viral interference.
Conclusions:
- Persistent SV5 infection in mouse L cells leads to a non-cytocidal state with high antigen expression and low infectious virus output.
- The observed viral interference suggests a role for defective interfering particles or endogenous oncornaviruses in maintaining the persistent infection.