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Related Experiment Videos

Plasma nitrate plus nitrite changes during continuous intravenous infusion interleukin 2

G Citterio1, F Pellegatta, G D Lucca

  • 1Divisione di Medicina II, IRCCS S. Raffaele, Milan, Italy.

British Journal of Cancer
|October 1, 1996
PubMed
Summary

Interleukin 2 (IL-2) cancer therapy increases nitric oxide (NO) levels, leading to hypotension in patients. Monitoring plasma nitrate and nitrite can predict vascular effects but not cardiac toxicity from IL-2 treatment.

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Area of Science:

  • Cardiovascular toxicology
  • Cancer immunotherapy
  • Nitric oxide metabolism

Background:

  • Interleukin 2 (IL-2) therapy in cancer patients is associated with cardiovascular toxicity.
  • Nitric oxide (NO) synthase induction and NO production are implicated in vascular leakage and myocardial contractility regulation.
  • The role of NO in IL-2-induced cardiovascular toxicity requires further investigation.

Purpose of the Study:

  • To evaluate plasma nitric oxide (NO) level changes during continuous intravenous infusion (CIVI) of IL-2 in advanced cancer patients.
  • To correlate plasma NO levels with cardiovascular side effects, specifically hypotension and cardiac toxicity.
  • To assess the dose-dependent pattern and cumulative effects of IL-2 on NO production.

Main Methods:

  • Ten advanced cancer patients received IL-2 (9 or 18 MIU m-2 day-1) via CIVI for 96 hours, repeated every 3 weeks.

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  • Plasma samples were collected at multiple time points during and after IL-2 cycles.
  • Plasma nitrate and nitrite concentrations were measured spectrophotometrically.

    • Main Results:

    • Plasma nitrate + nitrite levels significantly increased during IL-2 treatment, showing a dose-dependent pattern.
    • A progressive increase in plasma nitrate + nitrite levels was observed with increasing cumulative IL-2 dose across cycles.
    • Hypotension was observed in all patients, correlating with elevated mean and peak plasma nitrate + nitrite levels.

    Conclusions:

    • Plasma nitrate + nitrite levels during IL-2 therapy can estimate the degree of peripheral vascular relaxation and capillary leakage.
    • Elevated NO levels are associated with IL-2-induced hypotension, suggesting a role in vascular side effects.
    • IL-2-induced cardiac toxicity does not appear to correlate with plasma nitrate + nitrite levels, necessitating further research.