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Reference intervals of CD3, CD4, CD8, CD4/CD8, and absolute CD4 values in Asian and Non-Asian populations
R R Howard1, C S Fasano, L Frey
1Department of Pathology, University of California Medical Center, Davis at Sacramento, USA.
Cytometry
|September 15, 1996
Summary
This study found that Asian populations have lower T cell counts (CD3, CD4, CD8) and CD4/CD8 ratios compared to non-Asians. Race is a crucial factor for accurate immunophenotyping reference intervals.
Area of Science:
- Immunology
- Clinical Laboratory Science
Background:
- Accurate reference intervals for immunophenotyping are essential for diagnosing and monitoring various immune conditions.
- Previous studies have not sufficiently addressed potential racial variations in T cell subset reference ranges.
Purpose of the Study:
- To establish reference intervals for CD3, CD4, and CD8 T cells, including the CD4/CD8 ratio and absolute CD4 counts.
- To investigate whether race influences these reference intervals in Asian and non-Asian populations.
Main Methods:
- Utilized Coulter Clone/Cytostat reagents for immunophenotyping.
- Employed Coulter Q-Prep for sample lysis and Coulter Profile II flow cytometry for analysis.
- Compared T cell subset data between normal Asian and non-Asian cohorts.
Main Results:
- Asian populations exhibited a lower mean percentage of CD3 and CD4 T cells.
- A lower CD4/CD8 ratio and reduced absolute CD4 lymphocyte counts were observed in Asians.
- Significant differences suggest race impacts immunophenotyping reference values.
Conclusions:
- Race is an important consideration when establishing reference intervals for CD3, CD4, and CD8 immunophenotyping.
- Standard reference intervals may require ethnic-specific adjustments for optimal clinical interpretation.
- Further research is warranted to validate these findings across diverse ethnic groups.