A virus-inducible cucumber anionic peroxidase has a serological counterpart in different plant species

A highly specific rabbit antiserum raised against anionic peroxidase (PRX) purified from cucumber (Cucumis sativus L., cv. Laura) cotyledons infected with tobacco necrosis virus (TNV) was used to further investigate the antigenic relatedness of peroxidases in the selected plant species. Immunoblot analysis using native polyacrylamide electrophoresis (PAGE) revealed that the antiserum recognized only fast-moving anionic PRX isoenzymes and that cross- reacting protein bands were present in five plant species belonging exclusively to the Solanaceae family. There was no serological cross-reaction indicated in virus-infected plant species from three other dicotyledonous families (Amaranthaceae, Chenopodiaceae and Leguminosae). Marked qualitative, as well as quantitative differences in the degree of serological relatedness were apparent.