Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

SAS is amplified predominantly in surface osteosarcoma

S E Noble-Topham1, S R Burrow, K Eppert

  • 1Department of Pathology, Mount Sinai Hospital, Toronto, Ontario, Canada.

Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society
|September 1, 1996
PubMed
Summary

Gene amplification of the SAS gene is common in osteosarcoma, particularly surface subtypes. This finding suggests SAS plays a role in osteosarcoma development and subtype differences.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Canadian Surgery Forum: Abstracts of presentations to the Annual Meetings of the Canadian Association of Bariatric Physicians and Surgeons, Canadian Association of General Surgeons, Canadian Association of Thoracic Surgeons, Canadian Hepato-Pancreato-Biliary Association, Canadian Society of Surgical Oncology, Canadian Society of Colon and Rectal Surgeons, Vancouver, BC, Sept. 17-21, 2013.

Canadian journal of surgery. Journal canadien de chirurgie·2025
Same author

Cellular therapy and tissue engineering for cartilage repair.

Osteoarthritis and cartilage·2022
Same author

YAP/TAZ regulates the expression of proteoglycan 4 and tenascin C in superficial-zone chondrocytes.

European cells & materials·2020
Same author

Processing and properties of Na-doped porous calcium polyphosphates - Mechanical properties and in vitro degradation characteristics.

Journal of the mechanical behavior of biomedical materials·2019
Same author

The vestibulocochlear bases for wartime posttraumatic stress disorder manifestations.

Medical hypotheses·2017
Same author

TNF-α modulates genome-wide redistribution of ΔNp63α/TAp73 and NF-κB cREL interactive binding on TP53 and AP-1 motifs to promote an oncogenic gene program in squamous cancer.

Oncogene·2016

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Gene amplification, particularly of growth-promoting genes, contributes to human tumor development.
  • The SAS gene, located in the frequently altered q13-15 region of chromosome 12, is implicated in sarcomas.

Purpose of the Study:

  • To determine the frequency of SAS gene amplification in osteosarcoma.
  • To investigate the association between SAS gene amplification and osteosarcoma subtypes.

Main Methods:

  • Southern blot analysis was employed to detect gene amplification.
  • Quantitative polymerase chain reaction (qPCR) was used for precise quantification of SAS gene amplification.

Main Results:

  • SAS gene amplification was observed in 36% (10 of 28) of osteosarcoma cases.

Related Experiment Videos

  • All seven surface osteosarcomas exhibited SAS amplification, whereas only 13% (2 of 15) of intramedullary osteosarcomas showed amplification.

    • Conclusions:

    • The high frequency of SAS amplification in surface osteosarcomas suggests a significant role in their pathogenesis.
    • These findings indicate potential genetic distinctions between surface and high-grade intramedullary osteosarcomas.