Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Characterization of echovirus 22 variants

D Schnurr1, M Dondero, D Holland

  • 1Viral and Rickettsial Disease Laboratory, California Department of Health Services, Berkeley, USA.

Archives of Virology
|January 1, 1996
PubMed
Summary

Ten new enterovirus strains were identified as variants of echovirus 22 using molecular and serologic methods. These enterovirus variants, along with prototype strains, exhibited unusual heat stability.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Omega-3 polyunsaturated fatty acids for the management of metabolic syndrome in psychotic disorders: A systematic review and meta-analysis.

Schizophrenia research·2026
Same author

Commentary on: Inspiratory Muscle Training, with or without Pulmonary Rehabilitation, for COPD: A Critical Appraisal of a Cochrane Review.

Journal of the Association of Chartered Physiotherapists in Respiratory Care·2025
Same author

Surgical Timeliness in Acute Appendicitis: Evaluating Systemic and Demographic Factors Affecting Time to Theatre.

Irish medical journal·2025
Same author

Framework for classifying chemicals for repeat dose toxicity using NAMs.

Archives of toxicology·2025
Same author

Epidemiology of dog bite and strike related hospital admissions in Scotland, 1997 to 2022.

Public health·2025
Same author

Boron coordination change in barium borate melts and glasses and its contribution to configurational heat capacity, entropy, and fragility.

The Journal of chemical physics·2023

Area of Science:

  • Virology
  • Molecular Biology
  • Immunology

Background:

  • Enteroviruses are a significant cause of human disease.
  • Accurate identification and characterization of enterovirus strains are crucial for public health.
  • Existing diagnostic methods may not fully identify novel or variant strains.

Purpose of the Study:

  • To characterize ten presumptive enterovirus isolates that were resistant to neutralization by prototype antisera.
  • To determine the relationship of these isolates to known enterovirus types, specifically echovirus 22.
  • To investigate the phenotypic properties, including heat stability, of these novel strains.

Main Methods:

  • Molecular methods: Polymerase Chain Reaction (PCR) and viral protein fingerprinting.
  • Serologic methods: Neutralization assays using type-specific antisera and antisera raised against variant strains (prime strains).
  • Biologic assays: Determination of heat stability at 50°C for 1 hour.

Main Results:

  • Ten enterovirus isolates were identified as variants of echovirus 22 through molecular and serologic analyses.
  • Three isolates were confirmed as echovirus 22 prime strains, capable of neutralizing both the variant and prototype.
  • Seven of the ten variants, along with echovirus 22 and 23 prototypes, demonstrated heat stability, unlike typical enteroviruses.

Conclusions:

  • The study successfully identified and characterized novel variants of echovirus 22.
  • These findings expand the known diversity within the echovirus 22 serotype.
  • The observed heat stability suggests unique physicochemical properties of these enterovirus strains, potentially impacting their survival and transmission.

Related Experiment Videos