Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Gap junction structures. II. Analysis of the x-ray diffraction data

L Makowski, D L Caspar, W C Phillips

    The Journal of Cell Biology
    |August 1, 1977
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Sex related differences in therapy and outcome of patients with intermittent claudication in a real-world cohort.

    Atherosclerosis·2021
    Same author

    Lymphedema of the breast as a symptom of internal diseases or side effect of mTor inhibitors.

    Lymphatic research and biology·2012
    Same author

    Multi-wavelength anomalous diffraction using medium-angle X-ray solution scattering (MADMAX).

    Biophysical journal·2012
    Same author

    Characterisation of the learning curve of caesarean section.

    Archives of gynecology and obstetrics·2012
    Same author

    WAXS studies of the structural diversity of hemoglobin in solution.

    Journal of molecular biology·2011
    Same author

    STRUCTURE OF FILAMENTOUS BACTERIOPHAGE Pf1.

    Biophysical journal·2009
    Same journal

    Synergistic assembly, disassembly, and protection of complex forms of bundled F-actin.

    The Journal of cell biology·2026
    Same journal

    Recruitment and release of XPG during NER is controlled by pre- and post-incision factors and EXO1.

    The Journal of cell biology·2026
    Same journal

    Meiotic CENP-C supports centromere assembly and kinetochore recruitment in spermatogenesis.

    The Journal of cell biology·2026
    Same journal

    Phosphatidylserine and RhoB connect PI4P and PA metabolism to maintain plasma membrane identity.

    The Journal of cell biology·2026
    Same journal

    PIKfyve influences inter-organelle contacts with lysosomes to modulate the endoplasmic reticulum.

    The Journal of cell biology·2026
    Same journal

    Sequential changes in calcium transients during M phase regulate cardiomyocyte proliferation.

    The Journal of cell biology·2026
    See all related articles

    Researchers modeled gap junction structures using X-ray diffraction and electron microscopy. This revealed the spatial distribution of proteins, lipids, and water within these crucial cell communication channels.

    Area of Science:

    • Structural biology
    • Cell biology
    • Biophysics

    Background:

    • Gap junctions are essential for intercellular communication.
    • Understanding their molecular structure is key to deciphering cellular processes.

    Purpose of the Study:

    • To construct models of protein, lipid, and water distribution in gap junction structures.
    • To integrate X-ray diffraction, electron microscopy, and chemical data for a comprehensive structural analysis.

    Main Methods:

    • Analysis of X-ray diffraction data, including meridional and equatorial reflections.
    • Correction for specimen ordering and orientation effects.
    • Calculation of electron density distributions and pair-correlation functions.
    • Integration with electron microscopy and chemical data.

    Related Experiment Videos

    Main Results:

    • Determined the electron density distribution perpendicular to the gap junction plane.
    • Calculated a two-dimensional electron density projection onto the membrane plane.
    • Correlated structural variations in connexons with changes in lattice constant.
    • Distinguished variable from invariant parts of the junction structure.

    Conclusions:

    • Low-resolution three-dimensional models of gap junction structures were generated.
    • The study provides insights into the spatial organization of key molecular components within gap junctions.
    • Structural variability in gap junctions was identified and correlated with lattice parameters.