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Comparison of inoculation methods for testing enterococci by using vancomycin screening agar

J H Jorgensen¹, M L McElmeel, C W Trippy

¹Department of Pathology, University of Texas, Health Science Center, San Antonto 78284-7750, USA.

Journal of Clinical Microbiology

|November 1, 1996

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Detecting vancomycin resistance in Enterococcus species is crucial. Swab inoculation proved as effective as pipetting for identifying resistant strains, offering a convenient and cost-effective alternative for clinical laboratories.

Area of Science:

Clinical Microbiology
Antimicrobial Resistance
Infectious Diseases

Background:

Enterococcus species are significant nosocomial pathogens.
Vancomycin resistance in Enterococcus is a growing public health concern.
Accurate and efficient detection methods are essential for patient management.

Purpose of the Study:

To evaluate the efficacy of different inoculation methods for detecting vancomycin resistance in Enterococcus.
To compare the convenience and cost-effectiveness of swab inoculation versus pipetting methods.

Main Methods:

Screened 104 clinical Enterococcus isolates for vancomycin resistance.
Utilized three inoculation techniques: pipetting (10^5 or 10^6 CFU) and cotton swab (0.5 McFarland standard).
All methods used brain heart infusion agar supplemented with 6 µg/mL vancomycin.

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Main Results:

All three inoculation methods demonstrated equivalent performance in detecting vancomycin-nonsusceptible Enterococcus isolates.
Swab inoculation was identified as a convenient and less costly method compared to micropipetting.

Conclusions:

Swab inoculation is a viable and practical alternative for routine vancomycin resistance screening of Enterococcus in clinical settings.
This method supports efficient and economical antimicrobial susceptibility testing.