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Tissue protein washout in sheep lung lymph
R E Drake1, S Dhother, V M Oppenlander
1Department of Anesthesiology, University of Texas-Houston Medical School, USA.
Lymphology
|September 1, 1996
Summary
Lung lymph protein concentration may exceed filtrate levels due to tissue protein. This study quantifies lung tissue protein in lymph, offering a method to correct microvascular permeability measurements.
Area of Science:
- Physiology
- Pulmonary Medicine
- Biochemistry
Background:
- Lung lymph protein concentration (C1) can be influenced by factors beyond microvascular permeability.
- High microvascular filtration rates may lead to elevated C1 due to protein from lung tissue.
Purpose of the Study:
- To test the hypothesis that lung tissue protein contributes to lymph protein concentration at high filtration rates.
- To develop a method for estimating the concentration of protein originating from lung tissue in lymph.
Main Methods:
- Anesthetized sheep underwent increased microvascular filtration rates.
- The relationship between lung lymph protein concentration (C1) and plasma protein concentration (Cp) was determined.
- Data were extrapolated to estimate C1 at zero plasma protein concentration (Cp=0) to represent tissue protein concentration (Ct).
Main Results:
- The estimated lung tissue protein concentration in lymph (Ct) was 0.92 +/- 0.38 g/dL.
- This estimated Ct was significantly greater than zero (P < 0.05).
Conclusions:
- Lung tissue protein is present in lung lymph, particularly at high filtration rates.
- This tissue protein can introduce errors in studies of microvascular permeability using lung lymph.
- The developed estimation technique may enable corrections for tissue protein, improving accuracy in permeability studies.