Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Lipoxygenase activity in heart cells

E Breitbart1, Y Sofer, A Shainberg

  • 1Department of Life Sciences, Bar-Ilan University, Ramat Gan, Israel.

FEBS Letters
|October 21, 1996
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Exploring the prognostic impact of absolute lymphocyte count in patients treated with immune-checkpoint inhibitors.

BJC reports·2024
Same author

Should acrylic dentures be embedded with a radiopaque marker?

British dental journal·2023
Same author

Correction: Effects of a typical acute oral calcium load on arterial properties and endothelial function in healthy subjects.

European journal of clinical nutrition·2023
Same author

[Current dermatology guidelines in Germany-selected recommendations from 2021 and 2022].

Dermatologie (Heidelberg, Germany)·2023
Same author

Cannabis and orofacial pain: a systematic review.

The British journal of oral & maxillofacial surgery·2022
Same author

Dental Service and Resource Needs during COVID-19 among Underserved Populations.

JDR clinical and translational research·2022

This study investigated arachidonic acid metabolism in rat hearts and cardiomyocytes. Lipoxygenase (LOX) pathway products, 12-HETE and 15-HETE, were identified, revealing LOX activity in various cellular fractions.

Area of Science:

  • Biochemistry
  • Cardiovascular Biology
  • Lipid Metabolism

Background:

  • Arachidonic acid (AA) is a polyunsaturated fatty acid crucial for cellular signaling.
  • The lipoxygenase (LOX) pathway metabolizes AA into bioactive lipid mediators.
  • Understanding AA metabolism in cardiac cells is vital for cardiovascular health research.

Purpose of the Study:

  • To investigate arachidonic acid metabolism via the LOX pathway in rat hearts and cardiomyocytes.
  • To identify and characterize LOX products in different subcellular fractions.
  • To report the properties of LOX enzymes in these cardiac preparations.

Main Methods:

  • Utilized 1-[14C]arachidonic acid as a substrate for metabolic studies.
  • Separated cellular components into microsomal fractions and high-speed supernatants.

Related Experiment Videos

  • Employed thin-layer chromatography (TLC), enzyme-linked immunosorbent assay (ELISA), and radioimmunoassay (RIA) for product identification and quantification.

    • Main Results:

    • Detected LOX activity in rat heart microsomal fractions, high-speed supernatants, and cardiomyocyte supernatants.
    • Identified 12-hydroxyeicosatetraenoic acid (12-HETE) and 15-HETE as major LOX products across fractions via TLC.
    • ELISA confirmed 12-HETE formation in microsomal fractions and cardiomyocyte supernatant, while RIA indicated 15-HETE formation primarily in the ammonium sulfate (AS) pellet.

    Conclusions:

    • Established the presence and activity of the LOX pathway in rat cardiac cells and tissues.
    • Characterized the formation of specific LOX metabolites (12-HETE and 15-HETE) in distinct cellular fractions.
    • Provided insights into the localization and properties of LOX enzymes within the cardiac system.