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Ceramide induces apoptosis via CPP32 activation

N Mizushima1, R Koike, H Kohsaka

  • 1The First Department of Internal Medicine, Faculty of Medicine, Tokyo Medical and Dental University, Japan.

FEBS Letters
|October 21, 1996
PubMed
Summary
This summary is machine-generated.

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Cell-permeable ceramide activates CPP32, a key protease in apoptosis. This activation is essential for ceramide-induced cell death, indicating the sphingomyelin-ceramide pathway precedes CPP32.

Area of Science:

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Background:

  • Ceramide and interleukin-1beta converting enzyme (ICE) family proteases are crucial in apoptosis.
  • The precise relationship between ceramide and ICE family proteases requires further investigation.

Purpose of the Study:

  • To elucidate the relationship between ceramide and ICE family proteases in apoptosis.
  • To determine the role of CPP32 activation in ceramide-induced apoptosis.

Main Methods:

  • Treatment of Jurkat cells with cell-permeable ceramide.
  • Inhibition of CPP32 and ICE proteases using specific tetrapeptide inhibitors (DEVD-CHO and YVAD-CHO).
  • Analysis of apoptosis in wild-type and variant Jurkat cells with defective CPP32 activation.

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Main Results:

  • Cell-permeable ceramide induced cleavage and activation of CPP32, but not ICE.
  • Ceramide-induced apoptosis was inhibited by a CPP32-specific inhibitor (DEVD-CHO), but not by an ICE inhibitor (YVAD-CHO).
  • Jurkat cells with defective CPP32 activation exhibited resistance to both anti-Fas and ceramide-induced apoptosis.

Conclusions:

  • CPP32 activation is a necessary step for ceramide-induced apoptosis.
  • The sphingomyelin-ceramide pathway appears to function upstream of CPP32 activation in the apoptotic process.