Related Experiment Videos
The corneal epithelium after optisol-GS storage
T L Means1, D H Geroski, N L'Hernault
1Department of Ophthalmology, Emory University School of Medicine, Atlanta, Georgia, USA.
Cornea
|November 1, 1996
Summary
Human corneas stored in OptiSol-GS show minimal epithelial damage up to 6 days. Extended storage beyond 10 days results in significant epithelial cell loss and damage.
Area of Science:
- Ophthalmology
- Tissue Engineering
- Cell Biology
Background:
- Corneal preservation is crucial for transplantation success.
- OptiSol-GS is a common storage medium for human corneas.
- Understanding epithelial integrity during storage is vital.
Purpose of the Study:
- To assess the viability and ultrastructure of the corneal epithelium in human corneas stored in OptiSol-GS for extended periods (2-34 days).
Main Methods:
- Human corneas (n=64) stored in OptiSol-GS were analyzed.
- Calcein-AM/ethidium homodimer staining assessed epithelial cell viability.
- Scanning and transmission electron microscopy evaluated epithelial ultrastructure.
Main Results:
- Corneas stored up to 6 days exhibited minimal epithelial damage (20-25%).
- Storage for 7-10 days showed increased damage (30-35%).
- Corneas stored >10 days demonstrated significant epithelial damage (40-70%) and cell loss.
Conclusions:
- OptiSol-GS maintains corneal epithelium integrity for up to 6 days.
- Prolonged storage (>10 days) leads to substantial epithelial damage and cell loss.
- Storage duration significantly impacts corneal epithelial health in OptiSol-GS.