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Automated capillary electrochromatography: reliability and reproducibility studies

M T Dulay1, C Yan, D J Rakestraw

  • 1Department of Chemistry, Stanford University, CA 94305, USA.

Journal of Chromatography. A
|February 23, 1996
PubMed
Summary
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Routine capillary electrochromatography (CEC) using packed capillaries offers reliable separations. This method achieves high efficiency and reproducibility for neutral compounds, demonstrating its practical application.

Area of Science:

  • Analytical Chemistry
  • Separation Science

Background:

  • Capillary electrochromatography (CEC) combines features of capillary electrophoresis and liquid chromatography.
  • Developing routine and reproducible CEC methods is crucial for its wider adoption.

Purpose of the Study:

  • To demonstrate the routine application of capillary electrochromatography (CEC).
  • To evaluate the efficiency and reproducibility of CEC separations using packed capillaries.

Main Methods:

  • Incorporation of 75 microns I.D. capillaries packed with 3 microns octadecylsilica (ODS) particles into a commercial capillary zone electrophoresis (CZE) instrument.
  • Separation of a mixture of neutral compounds.

Main Results:

  • Achieved separation efficiency up to 181,000 plates/m in under 8 minutes.

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  • Demonstrated high reproducibility with capacity factors showing less than 2% variation over hundreds of consecutive runs spanning weeks.

    • Conclusions:

    • CEC separations can be performed reliably and routinely.
    • The developed method shows excellent performance for separating neutral compounds.